Invitrogen™ Proteinase K Powder

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.

Product Code	Brand	Quantity
10340065	Invitrogen™ AM2542	100 mg
10492645	Invitrogen™ AM2544	500 mg

Description

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.

Proteinase K Remains Active:
• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g. the presence of SDS or urea)
• In the presence of metal chelating agents (e.g. EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and Quality Testing: Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit Definition: One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Order Info

Shipping Condition: Dry Ice

Specifications

• Host Species: T. album
• Content And Storage: Store dry powder in desiccant at –20°C.
• Product Type: Proteinase K

• Packaging: Bottle
• Shipping Condition: Dry Ice
• Product Line: Ambion™

Safety and Handling

Product Identifier

• Proteinase K Powder

Signal Word

• Danger

Hazard Category

• Respiratory sensitiser Category 1

Hazard Statement

• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.

Supplemental information

• MIXTURE LIST-Contains : Proteinase

For Research Use Only. Not for use in diagnostic procedures.