Invitrogen™ MagMAX™-96 Total RNA Isolation Kit

The MagMAX-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue.

Brand:  Invitrogen™ AM1830

Product Code. 10764417

Includes: Kit contents: 1 Processing Plate and Lid; 11mL Lysis/Binding Solution Concentrate; 18mL Wash Solution 1 Concentrate; 55mL Wash Solution 2 Concentrate; 12mL RNA Rebinding Concentrate; 10mL Elution Buffer; 6mL MagMAX TURBO DNase Buffer; 1.1mL RNA Binding Beads; 1.1mL Lysis/Binding Enhancer; 110μL TURBO DNase. Contains enough reagents to isolate RNA from 96 samples.

Description

The MagMAX-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 μL of nuclease-free water, concentrating RNA from large, dilute samples.

Accommodates diverse sample types
The kit accommodates diverse biological samples and a broad range of cell and tissue input amounts. The kit is optimized for high-throughput isolation of RNA from 25 cells to 2 x 10⁶ cells, as well as small plant and mammalian tissue samples (up to 10mg). The protocol is fully amenable to automation. Detailed guidelines for general automation are included with the kit.

• Enhanced lysis/binding conditions to accommodate larger sample inputs
• Reagents for TURBO DNase™ treatment included to minimize DNA contamination and preserve RNA integrity
• Includes protocol for RNA isolation from plant tissues
• 96 reactions per kit suitable for moderate throughput
• Automated protocols, high-throughput compatible users

Accessory products
Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, and Wash Solution 2 Concentrate are components of the kit that may also be purchased separately. The Plant RNA Isolation Aid can be used in conjunction with the MagMAX-96 Total RNA Isolation Kit for the isolation of RNA from plant tissues.

Specifications

• Content And Storage: • 1 Processing Plate; room temperature
  • 11 mL Lysis/Binding Solution (concentrated); room temperature
  • 18 mL Wash Solution 1 (concentrated); room temperature
  • 55 mL Wash Solution 2 (concentrated); room temperature
  • 12 mL RNA Rebinding (concentrated); room temperature
  • 10 mL Elution Buffer; room temperature
  • 6 mL MagMAX TURBO DNase Buffer; room temperature
  • 1.1 mL RNA Binding Beads; 4°C
  • 1.1 mL Lysis/Binding Enhancer; -20°C
  • 110 μL TURBO DNase; -20°C
  
  
• Elution Volume: 50 μL
• Final Product Type: Total RNA
• For Use With (Equipment): KingFisher™ Duo Prime, KingFisher™ Flex, Automated Liquid Handling Systems
• Quantity: 96 Preps
• Starting Material Amount: Cells: ≤10⁶
  Plant: ≤10 mg
  Tissue: ≤5 mg

• Isolation Technology: Magnetic Bead
• Sample Type: Cells, Plants, Tissue
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
• Shipping Condition: Box 1: Dry Ice
  Box 2: Room Temperature
• Yield: ≤20 pg per cell

Safety and Handling

Product Identifier

• MagMAX-96 Total RNA Isolation Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 1 C
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P330-Rinse mouth.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P363-Wash contaminated clothing before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Proteinase, tritirachium album serine, Thiocyanic acid

For Research Use Only. Not for use in diagnostic procedures.