Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Brand:  Thermo Scientific™ AB1163A

Product Code. 11872043

Description

The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

• High Sensitivity & Specificity - The master mixes contain the patented hot start enzyme Thermo-Start DNA Polymerase
• Optimized buffer containing SYBR Green
• Reproducibility - All master mixes undergo extensive QC to ensure batch to batch consistency
• Broad dynamic range

Specifications

• Content And Storage: Contains:
  • 1 x 5 mL ABsolute QPCR SYBR Green ROX Mix (2X)
  • 100 μL MgCl₂ (1M)
  Sufficient for 400 x 25 μL reactions.
  
  Store at -20°C
• GC-Rich PCR Performance: High
• Polymerase: Taq DNA Polymerase
• For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
• Product Type: Real Time PCR SYBR Master Mix
• For Use With (Application): Gene Expression, Genotyping, siRNA Validation
• Concentration: 2X
• Sample Type: DNA, dsDNA

• Detection Method: SYBR
• PCR Method: qPCR
• Reaction Speed: Standard
• Product Line: ABsolute
• Sufficient For: 400 Reactions of 25 μL Each
• Compatibility: qPCR Cyclers
• No. of Reactions: 400 Reactions
• Volume: 5 mL

For Research Use Only. Not for use in diagnostic procedures.