2-Mercaptoethanol, Electrophoresis Grade, 98+%, Thermo Scientific Chemicals

Product Code	Brand	Quantity
15418219	Thermo Scientific Alfa Aesar J66742.30	250 g
15400647	Thermo Scientific Alfa Aesar J66742.0B	1000 g

Description

β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis. This product can also be used to form o-Phthalaldehyde-beta-mercaptoethanol (OPAME), a fluorogenic reagent shown to detect amino acids based on its reaction with primary amines. More recently OPAME has been used to distinguish between ssDNA/dsDNA and even single base mismatches in the hybridization of DNA. It is widely used for retarding oxidation of biological compounds in solution.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis. This product can also be used to form o-Phthalaldehyde-beta-mercaptoethanol (OPAME), a fluorogenic reagent shown to detect amino acids based on its reaction with primary amines. More recently OPAME has been used to distinguish between ssDNA/dsDNA and even single base mismatches in the hybridization of DNA. It is widely used for retarding oxidation of biological compounds in solution.

Solubility
Fully miscible in water. Soluble in alcohol, ether, benzene, and other organic solvents.

Notes
Handle under dry protective gas. Store in cool condition. Incompatible with air, moisture, oxidizing agents.

Chemical Identifiers

• CAS: 60-24-2
• Molecular Weight (g/mol): 78.13
• InChI Key: DGVVWUTYPXICAM-UHFFFAOYSA-N
• PubChem CID: 1567
• SMILES: OCCS

• Molecular Formula: C2H6OS
• MDL Number: MFCD00004890
• Synonym: 2-mercaptoethanol, mercaptoethanol, thioglycol, beta-mercaptoethanol, ethanol, 2-mercapto, 2-thioethanol, 2-hydroxy-1-ethanethiol, thioethylene glycol, 2-hydroxyethanethiol, 2-hydroxyethyl mercaptan
• ChEBI: CHEBI:41218

Specifications

• CAS: 60-24-2
• Color: Colorless
• Boiling Point: 156°C to 158°C
• Odor: Unpleasant
• Linear Formula: HSCH₂CH₂OH
• UN Number: UN2966
• Sensitivity: Air sensitive; Hygroscopic
• Synonym: 2-mercaptoethanol, mercaptoethanol, thioglycol, beta-mercaptoethanol, ethanol, 2-mercapto, 2-thioethanol, 2-hydroxy-1-ethanethiol, thioethylene glycol, 2-hydroxyethanethiol, 2-hydroxyethyl mercaptan
• InChI Key: DGVVWUTYPXICAM-UHFFFAOYSA-N
• IUPAC Name: 2-sulfanylethanol
• PubChem CID: 1567
• Formula Weight: 78.13
• Grade: Electrophoresis
• Chemical Name or Material: 2-Mercaptoethanol

• Melting Point: -100°C
• Density: 1.114 g/mL
• Flash Point: 73°C (163°F)
• Molecular Formula: C2H6OS
• MDL Number: MFCD00004890
• Beilstein: 773648
• Merck Index: 14,5869
• Solubility Information: Fully miscible in water. Soluble in alcohol,ether,benzene,and other organic solvents.
• SMILES: OCCS
• Molecular Weight (g/mol): 78.13
• ChEBI: CHEBI:41218
• Percent Purity: ≥98%
• Physical Form: Liquid

Safety and Handling

GHS H Statement
H301-H310-H318-H227-H315-H317-H335
Toxic if swallowed.
Fatal in contact with skin.
Causes serious eye damage.
Combustible liquid.
Causes skin irritation.
May cause an allergic skin reaction.
May cause respiratory irritation.

P210-P235-P260-P262-P264b-P270-P271-P272-P280-P301+P310-P302+P350-P304+P340-P305+P351+P338-P310-P311-P314-P330-P333+P313-P361-P363-P370+P378q-P501c

H227-H301+H331-H310-H315-H317-H318-H373

missing translation for 'dotInformation' : Transport Hazard Class: 6.1; Packaging Group: II; Proper Shipping Name: THIOGLYCOL

missing translation for 'einecsNumber' : 200-464-6

missing translation for 'rtecsNumber' : KL5600000

missing translation for 'tsca' : Yes

Recommended Storage : Ambient temperatures

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 2
• Acute toxicity Category 3
• LONG-TERM AQUATIC HAZARD Chronic 2
• ACUTE AQUATIC Acute 1
• Serious eye damage/eye irritation Category 1
• Reproductive toxicity Category 2
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H301-Toxic if swallowed.
• H310-Fatal in contact with skin.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H318-Causes serious eye damage.
• H331-Toxic if inhaled.
• H361f-Suspected of damaging fertility.
• H373-May cause damage to organs.
• H400-Very toxic to aquatic life.
• H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.

RUO – Research Use Only