GE Whatman Filtration for HPLC Sample Preparation
Accurate. Reliable. Fast
Simplify sample preparation with the Whatman™ regenerated cellulose membrane portfolio from GE Healthcare
- Reduce cost with well-designed devices – the regenerated cellulose (RC) family includes products for robotic sample handing, outside-of-lab sample preparation, certified method development, and working with hard-to-filter samples.
- Increase accuracy with reliable, repeatable results – The RC family is demonstrated to have extremely low levels of chemical extractables that could interfere with analysis. Products are available to retain a variety of particle sizes.
- Save time with multifunctional products – reduce time spent deciding which membrane type to use for your sample preparation. The RC membrane is broadly compatible with common HPLC sample solvents and mobile phases.
- Improve efficiency with ready-to-use products – RC products are supplied ready for use. The hydrophilic, low-protein binding RC membrane is suitable for a wide variety of HPLC applications.
Characteristics of common membranes
Solvent compatibility |
Qualities |
Membrane1 |
Aqueous |
Nonaqueous |
Low protein binding |
Low extractables |
| RC | +++ | +++ | +++ | +++ |
| CA | +++ | - | +++ | + |
| ME | +++ | + | + | - |
| CN | +++ | - | - | - |
| PVDF | +++ | +++ | +++ | + |
| PP | +++ | +++ | - | - |
| NYL | +++ | + | - | - |
| PES | +++ | + | +++ | +++ |
| PTFE | - | +++ | - | + |
| Anopore™ | +++ | + | +++ | +++ |
| PC | +++ | + | +++ | +++ |
| 1 | RC = regenerated cellulose; CA = cellulose acetate; ME = mixed ester; CN = cellulose nitrate; PVDF = polyvinylidene fluoride; PP = polypropylene; NYL = nylon; PES = polyethersulfone; PTFE = Polytetrafluoroethylene; PC = polycarbonate. |
| +++ | strong characteristic |
| + | weak characteristic |
| - | does not have characteristi |
The regenerated cellulose family
Mobile phase filtration
Whatman RC55 and GV 050/2 vacuum filtration unit for solvent filtration
Use the same material for mobile phase filtration and sample filtration to:
- Reduce variation of analysis
- Reduce rate of column clogging
- Enhance lifetime of column
If inline degassing is required, consider the Whatman Inline filter/de-gasser. Choose from two membrane options:
- Nylon – when mobile phase is > 20% aqueous
- Polypropylene – for non-aqueous solvents
Preventive care: Puradisc filters
Filtration of your samples is important as a preventive maintenance step for HPLC or UHPLC analysis. Keep unwanted particulate matter from entering the injector to increase column life, shorten run time, and optimize peak shape.
- Manufactured to a high standard in GE’s ISO 9001:2008 certified facilities
- RC available in 13 mm and 25 mm diameters; hold-up volume < 25 μL for 13 mm and < 100 μL for 25 mm
- Available in non-RC membrane types to support a variety of other applications
Typical data
Puradic Syringe Filters
4 mm diameter* |
13 mm diameter† |
25 mm diameter‡ |
30 mm diameter* |
|
| Housing | Polypropylene | Polypropylene | Polypropylene | Polycarbonate |
| Filtration area | 0.2 cm2 | 1.3 cm2 | 4.2 cm2 | 5.7 cm2 |
| Maximum pressure | 75 psi (5.2 bar) | 75 psi (5.2 bar) | 75 psi (5.2 bar) | 100 psi (6.9 bar) |
| Volume ‘hold up’ with air purge | < 10 μl | < 25 μl | < 100 μl | < 50 μl |
| Dimensions | 10.1 × 23.5 mm 19.5 × 7.7 mm (PVDF membrane only) |
16.3 × 19.8 mm | 22.9 × 28.4 mm | 26 × 34 mm |
| Weight (approx) | 0.55 g | 0.95 g | 2.7 g | 4.7 g |
| Volume throughput | up to 2 ml | up to 10 ml | up to 100 ml | up to 100 ml |
| Inlet connection | Female Luer lock | Female Luer lock | Female Luer lock | Female Luer lock |
| Outlet connection | Male Luer/tube tip | Male Luer/tube tip | Male Luer | Male Luer/tube tip |
| Sterilization§ | Autoclave at 121°C | Autoclave at 121°C+ | Autoclave at 121°C | Autoclaving not recommended |
| * | Diameters available for Puradisc only. |
| † | Data the same for Puradisc and Uniflo, except: Uniflo filtration area = 0.65 cm2; Max pressure = 67.5 psi (4.7 bar); Volume hold up is < 50 μl; dimensions = 19.6 × 16.9 mm |
| ‡ | Data the same for Puradisc and Uniflo, except: Uniflo filtration area = 4.9 cm2; Max pressure = 67.5 psi (4.7 bar); dimensions = 24.5 × 29.2 mm |
| § | Applies to nonsterile filters only. Do not autoclave sterile filters |
Certified quality for method development: SPARTAN filters
SPARTAN brand syringe filters are HPLC-certified for confidence and consistent results. Tested and certified for the absence of UV-absorbing substances (210 and 254 nm) with water, methanol, acetonitrile to ensure absence of interfering substances.
- Hydrophilic, low protein-binding membrane made of regenerated cellulose
- Excellent chemical resistance against the standard aqueous and organic HPLC solvents
- Tested and certified for the absence of UV-absorbing substances at wavelengths of 210 and 254 nm with water, methanol, and acetonitrile
- 13 mm diameter with Mini-Tip options
- 13 mm diameter with extremely low dead volume < 10 μL
High-particulate, challenging sample filtration: Whatman GD/X filters
Filter even the most difficult samples and use less hand pressure with GD/X syringe filters.
- Exceptional loading capacity and fast flow rates – prevents back pressure and clogging of membrane
- Graduated microfiber prefilter from 1 μm to 0.7 μm
- Higher flow rates (3×) compared to unprotected membranes
- Uses glass microfiber-based prefilters
For metals testing and other applications where glass-based compounds could interfere with analysis, we offer a related syringe filter (GD/XP), which uses polypropylene prefilters.
Typical data
Whatman GD/X Syringe Filters
Membrane |
GD/X 13 mm |
GD/X 25 mm |
| Housing | Polypropylene (pigment-free) | Polypropylene (pigment-free) |
| Filtration area | 1.3 cm2 | 4.6 cm2 |
| Maximum pressure | 100 psi (6.9 bar) | 75 psi (5.2 bar) |
| Volume “hold-up” full housing with air purge | 0.5 ml 50 μl (approx) | 1.4 ml 250 μl (approx) |
| Dimensions | 21.6 × 29.8 mm | 20.8 × 29.8 mm |
| Weight | 3 g (approx) | 3 g (approx) |
| Flow direction | Flow should enter from the inlet | Flow should enter from the inlet |
| Inlet connection | Female Luer lock | Female Luer lock |
| Outlet connection | Male Luer | Male Luer |
| Sterilization* | Autoclave at 121°C at 15 psi for 20 min | Autoclave at 121°C at 15 psi for 20 min |
| Glass microfiber prefiltration media | 100% borosilicate GMF 150: 1 μm GF/F 0.7 μm | 100% borosilicate GMF 150: 1 μm GF/F 0.7 μm |
| * | Applies to nonsterile filters only. Do not autoclave sterile GD/X filters. |
Typical data
GD/XP Syringe Filters
GD/XP 13 mm |
|
| Housing | Polypropylene (pigment-free) |
| Filtration area | 4.6 cm2 |
| Maximum pressure | 75 psi (5.2 bar) |
| Volume “hold-up” full housing | 1.4 ml with air purge 250 μl (approx) |
| Dimensions | 20.8 × 29.8 mm |
| Weight | 3 g (approx) |
| Flow direction | Flow should enter from the inlet |
| Inlet connection | Female Luer lock |
| Outlet connection | Male Luer |
| Sterilization† | Autoclave at 121°C at 15 psi for 20 min |
| Prefiltration media | PP 20 μm: 5 μm |
| † | Not recommended for nylon. |
Autosampler and workflow support: Mini-UniPrep filters
Whatman Mini-UniPrep Syringeless Filters - polypropylene or glass chambers
The Mini-UniPrep syringeless filters are compatible with most autosamplers.
- Easy-to-use design supports sample preparation outside of the lab if needed
- Process samples in one-third the time of traditional syringe filtration
- Replaces syringe, syringe filter, vial, and cap in one consumable
- Polypropylene or glass chamber options to prevent interference from chemical leaching
- Amber vials available for light sensitive samples
- Multi-compressors available for ease of use
- 12 × 33 mm vial can be used to filter up to 400 μL
Typical data
Mini-UniPrep and Mini-UniPrep G2 filter vials
Mini-UniPrep |
Mini-UniPrep G2 |
|
| Dimensions | Equivalent in size to 12 mm × 32 mm vials | Equivalent in size to 12 × 32 mm vials |
| Materials of construction | ||
| Chamber: | Polypropylene | Borosilicate glass |
| Plunger housing: | Polypropylene | Polypropylene |
| Plunger inner storage vial | N/A | Borosilicate glass/td> |
| Filter medium: | As specified | As specified |
| Septum: | Silicone with PTFE liner | Silicone with PTFE liner |
| Cap: | Polypropylene | Polypropylene |
| Maximum operating temp | 50°C (122°F) | 50°C (122°F) |
| Max. unfiltered sample capacity | 400 μL | 500 μL |
| Max. filtered sample capacity | 350 μL | 330 μL |
| Dead volume | 50 μL | 170 μL |
| Recommended minimum filtering volume | 100 μL | 220 μL placed in the chamber to obtain 50 μL in inner storage vial |
| Nominal force needed to compress | Approx. 8.2 kg (18 lbs) | Approx. 11.3 kg (25 lbs) |
| Autosampler needle height adjustment: | 3 mm from the bottom of Mini-UniPrep | 5 mm from the bottom of Mini-UniPrep G2 |
| Autosampler compatibility | Any autosampler that accommodates standard 12 × 32 mm profile vials | Any autosampler that accommodates standard 12 × 32 mm profile vials |