Protein Extraction and Purification
Thermo Scientific™ Pierce™ RIPA Buffer
Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit
Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay.
Triton™ X-100 (Electrophoresis), Fisher BioReagents™
CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO
Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™
CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
Invitrogen™ Magnetic Stand-96
Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories
Thermo Scientific™ Pierce™ DNase I
Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.
Thermo Scientific™ Pierce Protease Inhibitor Tablets
Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Thermo Scientific™ Triton X-100 Detergent Surfact-Ams™ Solution
Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.
GE Healthcare PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. GE Healthcare Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Merck™ BugBuster™ 10X Protein Extraction Reagent
Reagent for protein sample preparation
GE Healthcare HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Thermo Scientific™ Pierce™ Microcentrifuge Spin Cups and Spin Columns
Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™
CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
Thermo Scientific™ Halt™ Protease Inhibitor Cocktails
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
Invitrogen™ MagnaRack™ Magnetic Separation Rack
Holds up to 24 microcentrifuge tubes
GE Healthcare Gel Filtration Calibration Kits
Calibrate gel filtration columns simply, reliably. GE Healthcare Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.
Invitrogen™ 24 Deep-Well RB Block
Designed for experimental protocols that require volumes exceeding the capacity of conventional shallow well plates
Thermo Scientific™ Pierce™ Disposable Plastic Columns
Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.
Thermo Scientific™ Pierce™ Centrifuge Columns
Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.
GE Healthcare HisTrap™ FF Crude Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. GE Healthcare Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Merck™ Novagen™ Benzonase™ Nuclease, >99%
Degrades all forms of single strand, double stranded, linear, circular DNA RNA.
IBA GmbH™ 10x Buffer BXT; Strep-Tactin™XT Elution B.
IBA GmbH™ 10x Buffer BXT; Strep-Tactin™XT Elution B. is a ready-to-use buffer for elution of Strep-tagged proteins from Strep-Tactin™ XT.
GE Healthcare Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
GE Healthcare LabMate™ PD-10 Buffer Reservoirs
Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. GE Healthcare Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.
GE Healthcare HisTrap™ FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
GE Healthcare IgG Sepharose™ 6 Fast Flow Medium
Use for rapid purification of protein A and protein A fusion conjugates. IgG Sepharose™ 6 Fast Flow Medium achieves the highest flowrates.