Antibotics, Buffers and Supplements

Gibco™ Trypsin-EDTA (0.25%), Phenol red

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas TRYPSIN 0.25% EDTA

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Gibco™ Trypsin-EDTA (0.05%), Phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas TRYPSIN .05% EDTA

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Gibco™ TrypLE Express Enzyme (1X), No Phenol Red

Unlike porcine trypsin, free of animal-derived components TRYPLE EXPRESS

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Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test TRYPAN BLUE STAIN

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Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses 100ML Cell Recovery Solution

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Gibco™ Trypsin-EDTA (0.5%), No Phenol Red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas 100ML 0.5% Trypsin-EDTA (10x), no phenol redin plastic container, E-Z hold Store at -20C

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Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells INSULIN-TRANS-SEL-G, 100X

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Gibco™ Trypsin (2.5%), 10X Concentration

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas TRYPSIN, 2.5%, 10X

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Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media HEPES (1M) 100ML

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Gibco™ Sodium Pyruvate (100mM)

Growth supplement 100ML Sodium Pyruvate

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Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability 100 ML MEM NON-ESSENTIAL AMINO ACIDS SOLUTION10mM (100X), liquid 100mL RRR - pack at ambient

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Gibco™ ACK Lysing Buffer

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow ACK LYSING BUFFERACK Lysing Buffer is suitable for use in the

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Corning™ Trypan Blue

Can be used for variety cell counting methods requiring information on cell count and viability. 100 mL Trypan Blue Solution in PBS 0.4% (w/v)

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Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells. 5ML MatrigelhESC-Qualified Matrix, LDEV-Free

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Gibco™ L-Glutamine (200mM)

Ready-to-use stock solution that acts as a cell culture supplement 20ML L GLUTAMINE, 100X

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Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression. 5ML Matrigel Growth Factor Reduced (GFR) Basement

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Corning™ Cell-Tak Cell and Tissue Adhesive

Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis. 10MG Cell-Tak Cell and Tissue Adhesive

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Gibco™ 2-Mercaptoethanol (55mM)

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals 2 MERCAPTOETHANOL 1000X

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Gibco™ TrypLE Express Enzyme (1X), Phenol Red

Animal origin-free, recombinant enzyme X20 Tryple express kit with phenol red store at

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HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 5KG HEPES (Fine White Crystals) for Molecular Biology,

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Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression. 50ML Matrigel Basement Membrane Matrix, LDEV-Free

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Gibco™ Low Serum Growth Supplement (LSGS)

Low Serum Growth Supplement (LSGS) LSGS 10MLdermal fibroblasts + endothel cells

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Gibco™ Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix

Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix GELTREX HESC QUAL READY TO USE

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Gibco™ Pluronic F-68 Non-ionic Surfactant (100X)

A non-ionic surfactant used to control shear forces in suspension cultures PLURONIC

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Corning™ DMSO (Dimethyl Sulfoxide)

For cryopreservation of cells along with glycerol, to prevent damage to cell membrane during freezing 250 mL DMSO (Dimethyl Sulfoxide)

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Gibco™ Human Keratinocyte Growth Supplement (HKGS)

Human Keratinocyte Growth Supplement (HKGS) HKGS 5MLA sterile, concentrated (100X) solution intended

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Gibco™ Bacto™ Tryptone

Enzymatic digest of casein. Gibco™ Bacto™ Dehydrated Culture Media Additive: Tryptone is part of a large selection of dehydrated media. 500GR TRYPTONE

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Gibco™ Bacto™ Peptone

Enzymatic digest of protein 10KG PEPTONE

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Corning™ ITS Premix Universal Culture Supplement

Stimulates proliferation of a variety of cells under serum-reduced conditions. Corning™ ITS Premix Universal Culture Supplement contains insulin, human transferrin and selenous acid, the three most universally esential components of defined culture media. 5ML ITS Premix Universal Culture Supplement

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MES (Fine White Crystals), Fisher BioReagents

100GR MES monohydrate (Fine White Crystals),

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