Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Includes: Detection Reagent 1 and 2

Product Code	Brand	Quantity	Sufficient For
11517371	Thermo Scientific™ 80196X3	3 x 100 mL	30 Mini-gel blots, 3000cm2 of membrane

Description

Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.

Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.

Highlights:

• High sensitivity — detect targets down to the low-picogram level
• Long signal duration — sustained light output for as long as five hours
• More imaging options — X-ray, CCD or laser-based imagers
• More affordable — high quality and performance at a lower price than other suppliers' similar substrates

Specifications

• Product Type: ECL Western Blot Substrate
• Kit Contents: Detection reagent 1 and 2
• Content And Storage: 2°C to 8°C

Safety and Handling

Product Identifier

• Pierce ECL 2 Western Blotting Substrate

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 2
• Serious eye damage/eye irritation Category 2
• Carcinogenicity Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H319-Causes serious eye irritation.
• H351-Suspected of causing cancer.
• H335-May cause respiratory irritation.

Precautionary Statement

• P201-Obtain special instructions before use.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P235-Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : 1, 4-dioxane, ethanol