Thermo Scientific™ ClaSeek Library Preparation Kit, Illumina compatible

Generate Illumina™-compatible next-gen sequencing libraries from low sample input using a one-tube PCR-free method with optimized master mixes

Description

Thermo Scientific ClaSeek Library Preparation Kit, Illumina™ compatible, is designed for fast and convenient construction of PCR-free NGS fragment library from DNA sample input as low as 500 ng. In combination with the amplification protocol the kit also enables the generation of DNA fragment libraries from ultra-low 5 ng sample input.
The kit utilizes a highly efficient NGS-optimized library construction protocol combining end-conversion and adapter addition steps into a simple and convenient one-tube procedure. This minimizes unnecessary pipetting steps and reduces hands-on time, allowing for PCR-free library construction in less than 70 minutes. The kit is suitable for construction of 50, 300, 500 bp read-length Illumina™-compatible libraries and is validated for use on Illumina™ HiSeq™ and MiSeq™ sequencing platforms.

Highlights

• PCR-free - significantly minimized bias and improved coverage with amplification-free protocol
• Flexible - universal protocol for variable sample input (5 ng - 1 μg) and DNA fragment lengths
• Fast and Convenient - fewer cleanup steps, less hands-on time, optimized master mixes, one-tube end conversion procedure.

Applications
DNA fragment library preparation for downstream use in next generation sequencing workflow.

Includes

• ClaSeek End Conversion Master Mix, IL
• ClaSeek Ligation Mix, IL
• Water, nuclease-free