Invitrogen™ TGFBR2 Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15445143	Invitrogen™ EHTGFBR2	96 Tests
17195569	Invitrogen™ EHTGFBR2X5	5 x 96 Tests
18587854	Invitrogen™ EHTGFBR2X10

Description

The Human Transforming Growth Factor beta Receptor 2(TGFβR2) ELISA quantitates Hu TGFβR2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TGFβR2. Principle of the method The Human TGFβR2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Most mammalian cells express three abundant high affinity receptors, which can bind and be cross-linked to TGF beta: the type I (53 kDa), type II (65 kDa), and type III (100-280 kDa) receptors, based upon the molecular mass of the cross-linked products an.

Specifications

• Accession Number: P37173
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Specificity: This ELISA antibody pair detects human TGF-beta R2. Other species not determined yet.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 7048
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Apoptosis
• Synonym: AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TGFR-2, TGFbeta-RII
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 15 pg/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: AAT3,FAA3,LDS1B,LDS2,LDS2B,MFS2,RIIC,TAAD2,TGFR-2,TGFbeta-RII
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.