IBA Solutions For Life Science StarGate™ Upstream Fusion Vector pNFUSE-IBA

For upstream positioning of an inserted gene in the final Fusion Donor vector when linking two or more genes cloned in separate Donor Vectors using the StarGate™ transfer cloning system.

Brand:  IBA Solutions For Life Science 5-1634-001

Product Code. 15623728

Description

StarGate™ offers a simple cloning procedure for rapid and highly efficient subcloning of an arbitrary gene of interest (GOI) for use in functional expression, purification, detection, and immobilization or separation of recombinant proteins.

• Minimal extra modification of the gene due to short combinatorial sites
• Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
• Genes are cloned in a Donor Vector and then parallel-transfered into Acceptor Vectors that provide different genetic surroundings
• Easily connect two or more genes that are cloned in separate Donor Vectors using Fusion Vectors that are assembled by use of a one-tube reaction
• The GOI to be positioned upstream is transferred into a pNFUSE-IBA derivative, specifying the intergenic region (IR):
  • IRES11: Provides internal ribosomal entry site (IRES) from encephalomyocarditis virus for separate cap-independent translation of downstream cistron in mammalian cells
  • SD11: IR includes a Shine Dalgarno (SD) sequence for translation of downstream cistron at comparable level to the upstream cistron in bacterial cells
  • LINK11: Genes of interest are fused by a sequence encoding the 10 amino acid linker GlySerGlyGlyGlySerGlyGlyGlySer
  • LINK12: Genes of interest are fused by a sequence encoding the 15 amino acid linker GlySerGlyGlySerGlyGlyGlyGlySerGlyGlyGlyGlySer
• The GOIs to be positioned downstream are transferred into a pCFUSE-IBA11 vector (identical, irrespective of IR)

Specifications

• Format: Solution, liquid
• Description: Resistance: ampicillin
• pH: 8
• Storage Buffer: 20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μL pCFUSE-IBA11
• Concentration: 250 ng/μL
• Quantity: 5 μg
• For Use With (Application): Upstream positioning, fusing cloned genes of interest

• For Use With (Equipment): Stargate™ system derivatives
• Marker Gene: IR: A sequence encoding the 15 amino acid linker GlySerGlyGlySerGlyGlyGlyGlySerGlyGlyGlyGlySer
• Product Type: Fusion Vector
• Content And Storage: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Label or Dye: LINK12
• Color: Clear