IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.

Brand:  IBA Lifesciences 5-4263-001

Product Code. 15633768

Description

pPSG-IGA E. coli expression vectors:

pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.

As the T7 promoter is stronger than the tet promoter, pPSG-IBA vectors are recommended in cases where expression with the tet promoter does not lead to significant yields of the recombinant protein.

• High-level expression in E. coli by bacteriophage T7 promoter
• High-level transcription by T7 RNA polymerase in BL21 strains
• High-level expression of non-toxic proteins
• Induction by IPTG
• Suitable for in vitro transcription/translation

StarGate System

IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

• Minimal extra modification of the gene due to short combinatorial sites
• Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
• GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
• Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
• The final expression vector, the Destination Vector, is then placed into the respective host

Specifications

• Format: Liquid
• Description: pPSG-IBA 63
• pH: 8
• Product Type: Acceptor Vector for E. coli expression
• Content And Storage: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Concentration: 250 ng/μL
• Vector: Total length: 3469
• For Use With (Application): Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins

• For Use With (Equipment): StarGate™ System, E. Coli BL21(DE3)
• Inducing Agent: E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
• Promoter: T7
• Storage Buffer: 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
• Protein Tag: FLAG-tag for C-terminal
• Quantity: 5 μg
• Color: Clear