Invitrogen™ SPINT1 (HAI-1) Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15435133	Invitrogen™ EHSPINT1	96 Tests
17824220	Invitrogen™ EHSPINT1X10	10 x 96 Tests
17105569	Invitrogen™ EHSPINT1X5	5 x 96 Tests

Description

The Human SPINT1 (HAI-1) ELISA quantitates Hu SPINT1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SPINT1. Principle of the method The Human SPINT1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms.

Specifications

• Accession Number: O43278
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 6692
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Synonym: UNQ223/PRO256, HAI, HAI1, MANSC2
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 0.8 ng/mL
• Conjugate: Biotin
• Protein Family: Cell Structure & Organelle Proteins
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: HAI,HAI1,MANSC2
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.