Invitrogen™ Serpin A4 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHSERPINA4X5

Product Code. 17145559

Description

The Human Serpin A4 ELISA quantitates Hu Serpin A4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Serpin A4. Principle of the method The Human Serpin A4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.

Specifications

• Accession Number: P29622
• Assay Sensitivity: 0.12 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 5267
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plates, Adhesive Plate Covers, Assay Diluent Concentrate, Biotinylated Detection Antibody, SAV-HRP, Stop Solution, Standard, Wash Buffer, Chromogen
• Quantity: 5 x 96 Tests
• Research Discipline: Other Research
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 0.143 ng/mL to 35 ng/mL
• Calibration Range: 0.12 ng/mL to 35 ng/mL
• Conjugate: Biotin
• Protein Family: Proteases, Peptidases & Inhibitors
• Specificity: This ELISA pair antibody detects human Serpin A4. Other species not determined.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: KAL,KLST,KST,PI-4,PI4,kallistatin
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 1 μ;L; Serum, 1 μ;L; Supernatant, 100 μ;L
• Synonym: KAL, KLST, KST, PI-4, PI4, kallistatin
• Test Time: 1 hr. 20 min.