Invitrogen™ Sclerostin (SOST) Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15425133	Invitrogen™ EHSOST	96 Tests
17195559	Invitrogen™ EHSOSTX5	5 x 96 Tests

Description

The Human Sclerostin (SOST) ELISA quantitates Hu Sclerostin human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Sclerostin. Principle of the method The Human Sclerostin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

Specifications

• Accession Number: Q9BQB4
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CDD,DAND61,VBCH,SOST
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 15 pg/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA antibody pair detects human SOST. Other species not determined yet.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 50964
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Bone Research
• Synonym: UNQ2976/PRO7455/PRO7476, CDD1, VBCH, SOST
• Test Time: 1 hr. 20 min.