Thermo Scientific™ Pierce™ Silver Stain for Mass Spectrometry

Reversibly stain proteins in polyacrylamide gels to identify bands to excise; then destain the gel pieces for analysis by mass spectrometry.

Brand:  Thermo Scientific™ 24600

Product Code. 10404005

Description

Thermo Scientific™ Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis.

This silver staining kit for mass spectrometry (MS) bundles components of the high-performance Pierce Silver Stain Kit with optimized reagents to destain spots for subsequent in-gel tryptic digestion to recover peptide fragments for proteomics analysis. The resulting MS-compatible product and protocol deliver outstanding sensitivity and maintains favorable conditions for the high-yield recovery and identification (sequence coverage) of proteins by mass spectrometry. Silver staining of 2D gels is now an important intermediate step in a set of procedures that leads ultimately to identification of specific proteins in the proteome by mass fingerprinting methods. The Pierce Silver Stain protocol provides peak staining performance, flexibility, reliability and robustness for applications such as MALDI-MS.

Highlights:

• Sensitive and fast staining – the low-background, easy-to-use silver stain provides subnanogram sensitivity, detecting proteins at less than 0.25ng per spot in 30 minutes after fixing

• Flexible staining protocol – fix gel for 15 to 30 minutes or overnight without any effect on staining performance; stain for 1 to 30 minutes (typically 2 to 3 minutes)

• Robust – effective silver stain for even difficult-to-stain basic proteins, such as lysozyme (pI 10) and chymotrypsinogen A (pI 9.2), which are detectable at 0.2ng and 0.5ng, respectively

• Simple, trouble-free spot preparation – stained spots in excised gel-pieces are easily destained and made ready for tryptic digestion in one hour

• MS compatible – reagents and protocol are optimized to provide excellent performance in mass spectrometry following extraction of peptides from stained 1D or 2D gels (SDS-PAGE)

• Complete kit – contains all reagents necessary for staining and destaining before in-gel proteolysis and peptide recovery for analysis by mass spectrometry

• Convenient – kit-components are stable at room temperature; saves valuable refrigerator space and eliminates the need to equilibrate reagents before use

Includes:

Sensitizer, stain, developer and enhancer solutions for silver staining gels; Reagent solutions A and B for destaining excised gel pieces

Specifications

• Content And Storage: Kit Contents:
  • Silver Stain Sensitizer, 2 mL
  • Silver Stain Enhancer, 25 mL
  • Silver Stain Developer, 500 mL
  • Silver Stain, 500 mL
  • Silver Destain Reagent A, 4 mL
  • Silver Destain Reagent B, 14 mL
  
  Storage: Upon receipt store at ambient temperature
• Detection Method: Colorimetric
• Product Type: Silver Stain
• Quantity: 1 L

• Detection Location: In-Gel Detection
• Label or Dye: Silver
• Product Line: Pierce™
• Target Molecule: Protein

Safety and Handling

Product Identifier

• Pierce Silver Stain for Mass Spectrometry

Signal Word

• Warning

Hazard Category

• ACUTE AQUATIC Acute 1
• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H351-Suspected of causing cancer.
• H400-Very toxic to aquatic life.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P391-Collect spillage.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Formaldehyde, silvernitrate

For Research Use Only. Not for use in diagnostic procedures.