Thermo Scientific™ Pierce™ Silver Stain Kit

Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results.

Product Code	Brand	Quantity
10096113	Thermo Scientific™ 24612	1 L
30055046	Thermo Scientific™ 24612X3	3 x 1 L

Description

Thermo Scientific™ Pierce Silver Stain Kit is a rapid, ultra-sensitive and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results with many 1D and 2D gel types for both first-time and experienced users.

The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. In standard mini gels, proteins are detectable at greater than 0.25 ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.

Features:
• Sensitivity—detect proteins at 0.25 ng
• Protocol—gel staining can be performed within 5 min to 20 hrs, depending on desired sensitivity
• Gel-compatibility—staining performance is very good with a variety of commercial precast mini-gels and SDS-PAGE buffer-systems
• Workflow-compatible—mild chemical formulation ensures compatibility with mass spectrometry, sequencing, and other downstream procedures that depend on destaining and protein recovery

Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions, but the concentration is low enough that irreversible protein crosslinking is minimized.

Specifications

• Detection Location: In-Gel Detection
• Label or Dye: Silver
• Product Line: Pierce™

• Detection Method: Colorimetric
• Product Type: Stain Kit
• Target Molecule: Protein

Safety and Handling

Product Identifier

• pierce silver stain kit

Signal Word

• Danger

Hazard Category

• ACUTE AQUATIC Acute 1
• Carcinogenicity Category 2
• Germ cell mutagenicity Category 2
• Skin sensitiser Category 1

Hazard Statement

• H317-May cause an allergic skin reaction.
• H341-Suspected of causing genetic defects.
• H350-May cause cancer.
• H400-Very toxic to aquatic life.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P391-Collect spillage.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : formaldehyde, silvernitrate

For Research Use Only. Not for use in diagnostic procedures.