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Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
£138.00 - £718.00
Specifications
Product Type | Substrate |
---|---|
Detection Method | Chemiluminescent, Fluorescent |
Form | Liquid |
Membrane Compatibility | Nitrocellulose, PVDF |
Product Line | Pierce™ |
Product Code | Brand | Quantity | Sufficient For | Price | Quantity & Availability | ||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Sufficient For | Price | Quantity & Availability | ||||
11547910
|
Thermo Scientific™
32134 |
25 mL Kit | 250 sq. cm of membrane |
£138.00
25mL |
Estimated Shipment: 09-07-2024 Log in to see stock. |
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11527271
|
Thermo Scientific™
32132X3 |
300 mL Kit | 3000 sq. cm of membrane |
£718.00
Pack of 3 |
In Stock Log in to see stock. |
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11517271
|
Thermo Scientific™
32132 |
100 mL Kit | 10 mini-gel blots; 1000cm2 of membrane |
£301.00
100mL |
In Stock
Log in to see stock. |
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Description
Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.Highlights:
Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
Higher sensitivity — detect targets down to the low-picogram level
Longer signal duration — sustained light output for as long as five hours
More imaging options — X-ray, CCD or laser-based imagers
More affordable — high quality and performance at a lower price than other suppliers« similar substrates
Specifications
Substrate | |
Liquid | |
Pierce™ |
Chemiluminescent, Fluorescent | |
Nitrocellulose, PVDF | |
Low picogram to mid-femtogram |
For Research Use Only. Not for use in diagnostic procedures.