OPTIZYME™ M-MLV Reverse Transcriptase, Fisher BioReagents™

Brand:  Fisher BioReagents BP8104-1

Product Code. 12827162

Description

OPTIZYME™ M-MuLV RT is a genetically modified MLV RT that possesses RNA- and DNA-dependent polymerase activity.

Applications and Features:

• OPTIZYME M-MLV RT is used for efficient first strand cDNA synthesis for RT-PCR and real-time RT-PCR (up to 13kb)
• Generation of labeled cDNA probes for microarrays
• Synthesis of cDNA for cloning and expression
• Analysis of RNA by primer extension
• DNA labeling
• Optimum activity at 42°C (active up to 50°C)
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Supplied with:
5X OPTIZYME M-MLV RT Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 20mM MgCl₂, 50mM DTT
Source: E. Coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase
Quality Control:

• Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free
• Functionally tested in first strand cDNA and RT-PCR

Specifications

• Concentration: 200 U/μL
• pH: 8.3
• Storage Buffer: OPTIZYME™ M-MLV RT Buffer 5X : Tris HCl 250mM (8.3 pH at 25°C), KCl 250mM, MgCl2 20mM, DTT 50mM
• Source: E. coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus Reverse Transcriptase
• Product Type: OPTIZYME™ M-MLV Reverse Transcriptase

• Color: Colorless
• For Use With (Application): Generation of labeled cDNA probes for microarrays, synthesis of cDNA for cloning and expression, analysis of RNA by primer extension, DNA labeling, optimum activity at 42°C, incorporates modified Nucleotides
• Content And Storage: -20°C
• Quantity: 5000 U
• Form: Liquid

Safety and Handling

Product Identifier

• OPTIZYME