OPTIZYME™ AatII, Fisher BioReagents™

Brand:  Fisher BioReagents BP8041-1

Product Code. 11944802

Description

5'...G A C G T^C...3'
3'...C^T G C A G...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

• 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ ml BSA
• Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 50 - 100%
• Buffer 2: 20 - 50%
• Buffer 3: 0 - 20%
• Buffer 4: 100%
• Buffer 5: 0 - 20%

Storage Buffer:

• 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with AatII.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of AatII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Note: Activity decreases if reaction buffer pH is not between 7.5 and 8.0 at 25°C.

Compatible Ends: TaiI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: Completely overlaps - blocked

• GACGTC

(Cleavage is blocked)

EcoKI: Never overlaps - no effect

EcoBI: May overlap - no effect

• 5'...TGm6ACGTC(N)₄ TGCT...3'

3'...AC TGCAG(N)₄m6ACGA...5'

(No effect on cleavage)

Specifications

• Concentration: 10 U/μL
• Incubator Temperature: 37°C
• For Use With (Application): >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with AatII
• Content And Storage: Keep container tightly closed
• Cut Site: GACGT.C
• Form: Liquid

• Components: 25 to 50% Water, >50% Glycerin
• pH: 7.4
• Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
• Quantity: 300 U
• Product Type: AatII

Safety and Handling

Product Identifier

• OPTIZYME