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Invitrogen™ Ni-NTA Agarose
Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
Brand: Invitrogen™ R90110
Code : NEW
Additional Details : CAS Number : 53123-88-9 Weight : 0.10000kg Transport : UN number : 1170 Chem class : 3 Pack group : III
Description
- Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
- Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine
- One-step purification can be performed under both native and denaturing conditions
- Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications
- Guaranteed stable for 6 months when properly stored at 4°C
Specifications
Suspension | |
Affinity Column | |
Ni-NTA |
The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored. | |
Agarose | |
100 mL |
For Research Use Only. Not for use in diagnostic procedures.