Invitrogen™ Myeloperoxidase Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMMPOX10

Product Code. 18547924

Description

The Mouse Myeloperoxidase ELISA quantitates Ms Myeloperoxidase in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Myeloperoxidase. Principle of the method The Mouse Myeloperoxidase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Myeloperoxidase (MPO) is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains with a molecular weight range of 59 - 64 kDa, and two unglycosylated beta chains (MW ∽14 kDa) with total MW ∽150 kDa and a theoretical pI of 9.2. Traditionally, myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities such as periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently, it was shown that myeloperoxidase participates in the initiation and progression of cardiovascular disease. Myeloperoxidase possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers. Myeloperoxidase is part of the host defense system of polymorphonuclear leukocytes and myeloperoxidase is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN (polymorphonuclear leukocytes), MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Specifications

• Accession Number: P11247
• Assay Sensitivity: 600 pg/mL
• Description: Myeloperoxidase ELISA Kit, 96 Assays, 1 Kit, Mouse Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Myeloperoxidase (MPO) in Samples Using a Microplate Reader
• Assay: Solid-phase sandwich ELISA
• Detection Method: Colorimetric
• Protein Family: Other Proteins
• Specificity: This ELISA antibody pair detect mouse MPO. Other species not determined yet
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: mKIAA4033
• Interassay CV: <12%
• Kit Contents: Assay Diluent concentrate
• Quantity: 10 x 96 Tests
• Research Discipline: Other Research
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 0.61 to 150 ng/mL
• Calibration Range: 0.6 ng/mL to 150 ng/mL
• Detectable Analytes: Myeloperoxidase (MPO), myeloperoxidase (MPO), mouse MPO
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma
• Sufficient For: 96 Determinations
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 17523
• Host Species: Mouse
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 µL; Serum, 50 µL; Supernatant, 100 µL
• Synonym: RP23-449P23.5, mKIAA4033
• Test Time: 1 hr. 20 min.