Invitrogen™ SDF-1 alpha/CXCL12 Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMCXCL12

Product Code. 15445253

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: P40224
• Sample Type: Supernatant
• Assay Range: 9.38-600 pg/mL
• Conjugate: Biotin
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Product Type: ELISA Kit
• Regulatory Status: RUO
• Sample Size: 100 μL per sample after dilution
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3
• Sufficient For: 96 Assays
• Total Assay Time: 4 hr. 45 min.
• Assays: Solid-phase sandwich ELISA
• Host Species: Mouse
• Research Discipline: Immunology
• Synonym: Pbsf, Scyb12, Sdf1, Tlsf, Tpar1
• Interassay CV: <12%
• Label or Dye: HRP

• Quantity: 96 Tests
• Target Species: Mouse
• Calibration Range: 3pg/mL to 600pg/mL
• Description: SDF-1 alpha CXCL12 ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Streptavidin-HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of SDF-1 alpha CXCL12 in Samples Using a Microplate Rea
• Gene ID (Entrez): 20315
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-mouse SDF-1 alpha precoated 96-well strip plate, lyophilized recombinant mouse SDF-1 alpha standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5
• Packaging Quantity: 1 Kit
• Sensitivity: 3 pg/mL
• Sample Volume: Plasma, 50 μL|Serum, 50 μL|Supernatant, 100 μL
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.
• Target: Mouse
• Detectable Analytes: SDF-1 alpha CXCL12, mouse SDF-1
• Protein Family: Chemokine & Receptors
• Gene Symbols: Cxcl12
• Detection Method: Colorimetric
• Intraassay CV: <10%