Invitrogen™ IFN alpha Mouse ELISA Kit

For the quantitative detection of mouse IFN-alpha.

Brand:  Invitrogen™ BMS6027TWO

Product Code. 15944214

Includes:

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IFN-alpha
• Biotin-Conjugate anti-mouse IFN-alpha monoclonal antibody
• Streptavidin-HRP
• Mouse IFN-alpha Standard lyophilized, 4000pg/mL upon reconstitution
• Calibrator Diluent
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution (tetramethyl-benzidine)
• Stop Solution (1M Phosphoric acid)
• Blue-Dye
• Green-Dye
• Red-Dye
• Adhesive Films

Description

The Mouse Interferon alpha (Ms IFNα) ELISA quantitates Ms IFNα in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IFNα. Principle of the method The Mouse IFNα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

Specifications

• Accession Number: P01572
• Assay Sensitivity: 7.48 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Ifa1
• Intraassay CV: 3.7%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 31.3 to 2000 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 15962
• Interassay CV: 7.2%
• Kit Contents: Chromogen, Standard, Adhesive Plate Covers, SAV-HRP, Wash Buffer, Pre-coated 96 well plate, Sample Diluent, Biotinylated Detection Antibody, Assay Buffer concentrate, Stop Solution
• Quantity: 2 x 96 tests
• Sample Volume: Plasma, 50 uL; Serum, 50 uL; Supernatant, 50 uL
• Synonym: Alpha interferon, IFN leukocytic, LeIF D
• Test Time: 1 hr. 20 min.

For Research Use Only