Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Useful with a variety of fluorescence-detection methodologies

Brand:  Invitrogen™ L3224

Product Code. 10237012

Description

The LIVE/DEAD Viability/Cytotoxicity Kit is a quick and easy two-color assay to determine viability of cells in a population based on plasma membrane integrity and esterase activity. The kit can be used in flow cytometry, fluorescence microscopy, and with fluorescence microplate readers.

• Faster, Safer, more sensitive
• Less expensive

Easily Use with a Wide Variety of Techniques and Cell Types
Ubiquitous intracellular esterase activity and an intact plasma membrane are distinguishing characteristics of live cells. The LIVE/DEAD Viability/Cytotoxicity Kit quickly discriminates live from dead cells by simultaneously staining with green-fluorescent calcein-AM to indicate intracellular esterase activity and red-fluorescent ethidium homodimer-1 to indicate loss of plasma membrane integrity. It is adaptable to most eukaryotic cells where cytotoxic conditions produce these cellular effects. The assay is useful with a variety of fluorescence-detection methodologies.

Sensitive, Safe, and Efficient
The LIVE/DEAD Viability/Cytotoxicity Kit is more sensitive than Trypan blue exclusion, a commonly used method for live/dead cell discrimination. The cost-effective LIVE/DEAD Viability/Cytotoxicity Kit is highly sensitive due to the bright fluorescence of both dyes upon interacting with either live (for calcein-AM) or dead (for ethidium homodimer-1) cells. Background levels are low due to the fact that both dyes are virtually non-fluorescent prior to interacting with cells.

LIVE/DEAD Assays Available for a Broad Range of Applications
A selection of Invitrogen LIVE/DEAD Viability Assays is offered for mammalian cells, bacteria, yeast and fungi, as well as Fixable Dead Cell Stain Kits for use in intracellular staining for flow cytometry. All LIVE/DEAD assays provide quick, positive discrimination between viable and non-viable cells.

Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cellular Imaging, Cellular Toxicology Assays, Drug Discovery and Development, Flow Cytometry, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, High-Content Screening (HCS), Immunofluorescence (IF), Immunofluorescence Staining and Detection, Target-Based ADME/Tox Assays

Order Info

Shipping Condition: Room temperature

Specifications

• Cell Type: Mammalian Cells
• Content And Storage: Store in freezer at -5°C to -30°C and protect from light.
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Microplate Reader
• Dye Type: Other Label(s) or Dye(s)
• Excitation Wavelength Range: 494, 528 nm
• Product Line: LIVE/DEAD™
• Shipping Condition: Room Temperature

• Color: Green, Red
• Description: LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
• For Use With (Application): Viability Assay
• Product Type: Viability/Cytotoxicity Kit
• Emission: 517, 617 nm
• Format: Tube(s), 96-well plate, Slide(s)
• Quantity: 1 kit

Safety and Handling

Product Identifier

• LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells

Signal Word

• Warning

Hazard Category

• Carcinogenicity Category 2

Hazard Statement

• H351-Suspected of causing cancer.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 5,5-[Ethylenebis(iminotrimethylene)]bis[3,8-diamino-6-phenylphenanthridinium] dichloride dihydrochlo

For Research Use Only. Not for use in diagnostic procedures.