Invitrogen™ Insulin Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMINS

Product Code. 15485303

Description

The Mouse Insulin ELISA quantitates Ms Insulin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Insulin. Principle of the method The Mouse Insulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

Specifications

• Accession Number: P01325
• Assay Sensitivity: 5μIU/mL
• Description: Insulin ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Hormones and Receptors Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Insulin in Samples
• Assay: Solid-phase sandwich ELISA
• Detection Method: Colorimetric
• Protein Family: Hormones & Receptors
• Specificity: This ELISA pair antibody detects mouse, human, rat, porcine and bovine Insulin.
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Ins-1,Ins2-rs1
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-Rat insulin precoated 96-well strip plate, lyophilized recombinant rat insulin standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent C, assay dilue
• Quantity: 96 Tests
• Research Discipline: Endocrinology
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 6.25-400μIU/mL
• Calibration Range: 5μIU/mL to 400μIU/mL
• Detectable Analytes: Mouse, human, rat, porcine and bovine insulin
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 Assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 16333
• Host Species: Mouse
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Synonym: Ins-1, Ins2-rs1
• Test Time: 1 hr. 20 min.