Invitrogen™ IL-13RA2 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHIL13RA2X5

Product Code. 17125479

Description

The Human Interleukin 13 Receptor Alpha 2 (Hu IL-13 RA2) ELISA quantitates Hu IL-13 RA2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-13 RA2. Principle of the method The Human IL-13 RA2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is closely related to Il13RA1, a subuint of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13. [provided by RefSeq, Jul 2008].

Specifications

• Accession Number: Q14627
• Assay Sensitivity: 0.4 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 3598
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plates, Biotinylated Detection Antibody, Standard, SAV-HRP, Adhesive Plate Covers, Stop Solution, Wash Buffer, Chromogen, Assay Diluent Concentrate
• Quantity: 5 x 96 Tests
• Research Discipline: Signal Transduction
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 0.41 ng/mL to 100 ng/mL
• Calibration Range: 0.4 ng/mL to 100 ng/mL
• Conjugate: Biotin
• Protein Family: Cytokines & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD213A2,CT19,IL-13 R,IL13BP
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L
• Synonym: CD213A2, CT19, IL-13 R, IL13BP
• Test Time: 1 hr. 20 min.