Invitrogen™ IGF1R Human ELISA Kit

Sandwich ELISA Kit

Marke:  Invitrogen™ EHIGF1RX5

Artikelnummer. 17115469

Beschreibung

The Human Insulin-like Growth Factor 1R (Hu IGF1R) ELISA quantitates Hu IGF1R in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IGF1R. Principle of the method The Human IGF1R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Insulin growth factor 1 receptor belongs to the family of tyrosine receptor kinases. It is involved in activation of various substrates including IGF and neurotensin, regulation of metabolism, cell division, maintenance, and inflammation response. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation. This antibody recognizes the beta-subunit of IGF-1R.

Spezifikation

• Accession Number: P08069
• Assay Sensitivity: 6 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 3480
• Interassay CV: <12%
• Kit Contents: SAV-HRP, Assay Diluent Concentrate, Standard, Chromogen, Adhesive Plate Covers, Biotinylated Detection Antibody, Wash Buffer, Pre-coated 96 well plates, Stop Solution
• Quantity: 5 x 96 Tests
• Research Discipline: Signal Transduction
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 8.23 pg/mL to 6000 pg/mL
• Calibration Range: 6 pg/mL to 6000 pg/mL
• Conjugate: Biotin
• Protein Family: Hormones & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD221,IGFIR,IGFR,JTK13
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L
• Synonym: CD221, IGFIR, IGFR, JTK13
• Test Time: 1 hr. 20 min.