Invitrogen™ IgE Mouse Uncoated ELISA Kit

Uncoated ELISA Kit

Brand:  Invitrogen™ 88-50460-77

Product Code. 15530907

Includes:

• Capture Antibody Concentrate
• Detection Antibody Concentrate
• mouse IgE isotype control (standard), lyophilized, 500ng/mL upon reconstitution
• Phosphate Buffered Saline Concentrate (PBS)
• Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
• Pre-titrated Streptavidin-HRP
• Tetramethylbenzidine (TMB) Substrate Solution
• 96 Well Plate included with product Cat. #’s ending in suffixes -22, -76, -86

Description

The Mouse Immunoglobulin E (IgE) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IgE. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.

Specifications

• Assay Range: 4-250 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
• Quantity: 20 x 96 Tests
• Sample Volume: Plasma, 10 μL; Serum, 10 μL; Supernatant, 50 μL
• Synonym: Immunoglobulin E
• Test Time: 1 hr. 45 min.

• Assay Sensitivity: 4 ng/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 24 hr. 30 min.