Invitrogen™ Human Thyroid Peroxidase ELISA Kit

ELISA

Brand:  Invitrogen™ EHTPO

Product Code. 15485163

Description

The Human Thyroid Peroxidase ELISA quantitates Hu Thyroid Peroxidase in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Thyroid Peroxidase. Principle of the method The Human Thyroid Peroxidase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Thyroid Peroxidase is a gene that encodes for a membrane-bound glycoprotein, which functions as an enzyme and plays a crucial role in the proper functioning of the thyroid gland. This protein is responsible for the iodination of tyrosine residues in thyroglobulin and the formation of phenoxy-ester between pairs of iodinated tyrosines, which leads to the production of thyroid hormones such as thyroxine and triiodothyronine. Mutations in this gene are known to cause several disorders related to thyroid hormone production, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Several transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants remains undetermined. Diseases associated with TPO include Thyroid Dyshormonogenesis 2A and Congenital Hypothyroidism.

Specifications

• Accession Number: P07202
• Assay Sensitivity: 0.3 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 Assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 7173
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Endocrinology
• Storage Requirements: 2°C to 8°C
• Target: Thyroid Peroxidase
• Total Assay Time: 4 hrs 45 mins

• Assay Range: 0.41 to 100 ng/mL
• Calibration Range: 0.3 ng/mL to 100 ng/mL
• Conjugate: HRP
• Protein Family: Proteases, Peptidases & Inhibitors
• Specificity: This ELISA pair antibody detects human TPX. Other species not determined.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: MSA,TDH2A,TPX
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Synonym: MSA, TDH2A, TPX
• Test Time: 1 hrs 20 mins