Invitrogen™ Human LAIR1 ELISA Kit

Human LAIR1 quantitates human LAIR1 in serum, plasma, supernatant.

Brand:  Invitrogen™ EH291RBX5

Product Code. 17155059

Description

Human LAIR1 quantitates human LAIR1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human LAIR1.

LAIR1 (CD305) functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.

Specifications

• Accession Number: 5133400O11Rik; BB115266; CD305; D7Bwg0421e; immunoglobulin heavy chain variable region; Lair1; LAIR-1; leukocyte associated immunoglobulin like receptor 1; leukocyte-associated Ig-like receptor 1; leukocyte-associated Ig-like receptor-1; leukocyte-associated immunoglobulin-like receptor 1; rLAIR1
• Assay Sensitivity: 0.6 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: LAIR1
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min

• Assay Range: 0.6 to 130 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): Q6GTX8
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 5 x 96 Tests
• Sample Volume: Supernatant, 50 μL; Serum, 50 μL; Plasma, 50 μL
• Target: LAIR1 (CD305)
• Total Assay Time: 4 hr 45 min