Invitrogen™ Human IL-12 p70 Uncoated ELISA Kit with Plates

ELISA

Product Code	Brand	Quantity
15591057	Invitrogen™ 88-7126-22	2 x 96 Tests
15501067	Invitrogen™ 88-7126-76	20 x 96 Tests
15521067	Invitrogen™ 88-7126-86	10 x 96 Tests

Description

The Human Interleukin 12 p70 (IL-12 p70) Uncoated ELISA Kit contains pre-matched antibody pairs,plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-12 p70. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

Specifications

• Accession Number: P29459,P29460
• Assay Sensitivity: 4 pg/mL
• Product Type: ELISA
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: AA690181,IL-15 RA,AI503618,IL-15
• Label or Dye: HRP
• Sample Volume: Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
• Synonym: Interleukin-12, IL12, IL23
• Test Time: 1 hrs 45 mins

• Assay Range: 4 to 500 pg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 3592, 3593
• Kit Contents: Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: IL-12 p70
• Total Assay Time: 24 hrs 30 mins