Invitrogen™ Human Alpha-Galactosidase A/GLA ELISA Kit

Human Alpha-Galactosidase A/GLA quantitates human Alpha-Galactosidase A/GLA in serum, plasma, supernatant.

Brand:  Invitrogen™ EH21RBX5

Product Code. 17134979

Description

Human Alpha-Galactosidase A/GLA quantitates human Alpha-Galactosidase A/GLA in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Alpha-Galactosidase A/GLA.

This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.

Specifications

• Accession Number: Agalsidase; agalsidase alfa; Ags; Alpha-D-galactosidase A; alpha-D-galactoside galactohydrolase; alpha-D-galactoside galactohydrolase 1; alpha-gal A; alpha-galactosidase A; GALA; galactosidase alpha; galactosidase, alpha; GLA; melibiase
• Assay Sensitivity: 45 pg/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: GLA
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min

• Assay Range: 45 to 10,000 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): P06280
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 5 x 96 Tests
• Sample Volume: Serum, 50 μL; Plasma, 50 μL; Supernatant, 50 μL
• Target: alpha Galactosidase
• Total Assay Time: 4 hr 45 min