Thermo Scientific Chemicals HEPES-buffered saline, pH 7.0 (5X)

Product Code	Brand	Quantity
15463469	Thermo Scientific Chemicals J60753.AK	250 mL
15473469	Thermo Scientific Chemicals J60753.AP	500 mL

Description

HEPES is widely used in cell culture. HEPES is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization. N-2-hydroxyethylpiperazine-N-2?-ethanesulfonic acid (Hepes) has been tested as an alternative to tris-(hydroxymethyl)aminoethane (Tris) for hydrogen-ion buffering of algal cultures. Separation of human platelets from plasma proteins including factor VIII VWF by a combined albumin gradient-gel filtration method by using HEPES buffer. May be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
HEPES is widely used in cell culture. HEPES is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization. N-2-hydroxyethylpiperazine-N-2′-ethanesulfonic acid (Hepes) has been tested as an alternative to tris-(hydroxymethyl)aminoethane (Tris) for hydrogen-ion buffering of algal cultures. Separation of human platelets from plasma proteins including factor VIII VWF by a combined albumin gradient-gel filtration method by using HEPES buffer. May be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions.

Solubility
Soluble in H2O: 1 M at 20°C, clear, colorless

Notes
Contains 125mM HEPES and 750mM sodium chloride, at pH 7.0

Chemical Identifiers

• CAS: 7365-45-9
• Molecular Weight (g/mol): 238.30
• InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N
• ChEBI: CHEBI:42334
• SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

• Molecular Formula: C8H18N2O4S
• MDL Number: MFCD00006158
• PubChem CID: 23831
• IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

Specifications

• CAS: 7365-45-9
• pH: 7.0
• Molecular Formula: C8H18N2O4S
• MDL Number: MFCD00006158
• InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N
• IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
• PubChem CID: 23831
• Chemical Name or Material: HEPES-buffered saline

• Concentration: 5X
• Physical Form: Liquid
• Odor: Odorless
• Solubility Information: Soluble in H2O: 1 M at 20°C,clear,colorless
• SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
• Molecular Weight (g/mol): 238.30
• ChEBI: CHEBI:42334

Safety and Handling

missing translation for 'tsca' : Yes

Recommended Storage : Ambient temperatures

RUO – Research Use Only