Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit

The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.

Brand:  Invitrogen™ A24372

Product Code. 15396351

Includes:

• 1 bottle Cell Lysis Buffer
• 1 tube Proteinase K
• 1 tube PCR Supermix
• 1 tube Water
• 1 tube Detection Enzyme
• 1 tube Detection Reaction Buffer
• 1 tube Control Template & Primers

Description

The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation. When using genome editing tools to obtain targeted mutations, it is necessary to determine the efficiency at which these nucleases cleave the target sequence, particularly prior to proceeding to the more laborious and expensive processes of cloning and sequencing.

The GeneArt genomic cleavage detection kit contains all of the reagents required prior to gel analysis. A sample of the edited cell population is used as a direct PCR template for amplification with primers specific to the targeted region. The PCR product is then denatured and re-annealed to produce heteroduplex mismatches where double strand breaks have occurred, resulting in indel introduction. These mismatches are recognized and cleaved by the detection enzyme. This cleavage is both easily detectable and quantifiable using gel analysis.

• Easy—no genomic DNA isolation, direct PCR amplification
• Rapid—4 hour total processing time
• Quantative—gel band density directly correlated to target indel introduction

Recommended Storage

Store all at -5 to -30°C

Specifications

• Content And Storage: Contains:
  
  • 1 bottle Cell Lysis Buffer
  • 1 tube Proteinase K
  • 1 tube PCR Supermix
  • 1 tube Water
  • 1 tube T7E1 Detection Enzyme
  • 1 tube T7E1 Detection Reaction Buffer
  • 1 tube Control Template & Primers
  
  Store all components at -5° to -30°C.
• Form: Liquid
• Polymerase: Taq Polymerase
• Technique: CRISPR-Cas9, TAL Effector Nuclease
• Quantity: 20 reactions
• Sufficient For: 20 Reactions

• Detection Method: Primer-probe
• Format: Kit
• Reaction Speed: Fast
• Product Type: Genomic Cleavage Detection Kit
• Recognition Site: INDEL
• No. of Reactions: 20 Reactions

Safety and Handling

Product Identifier

• GeneArt Genomic Cleavage Detection Kit

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1

Hazard Statement

• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Poly(oxy-1,2-ethanediyl) .alpha.-[4-(1,1,3,3-tetramethylbutyl)phenyl]-.omega.-hydroxy, Proteinase

For Research Use Only. Not for use in diagnostic procedures.