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Invitrogen™ FGF19 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHFGF19

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Product Code. 15414993

  • £544.00 / 1 set
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Description

Description

The Human Fibroblast Growth Factor 19 (Hu FGF19) ELISA quantitates Hu FGF19 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF19. Principle of the method The Human FGF19 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Fibroblast growth factor-1 (FGF-1), also designated acidic FGF, and fibroblast growth factor-2 (FGF-2), also designated basic FGF, are members of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (hst/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10-FGF-23. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

O95750
30 pg/mL
Solid-phase sandwich ELISA
ELISA Kit
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
9965
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Neurobiology
2°C to 8°C
Human
4 hr. 45 min.
32.77-8000 pg/mL
30pg/mL to 8000pg/mL
Biotin
Growth Factors & Receptors
This ELISA antibody pair detects human FGF-19. Other species not determined yet.
Human
Colorimetric Microplate Reader
FGF19
<10%
HRP
RUO
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
UNQ334/PRO533
1 hr. 20 min.
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