Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kits

Kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane.

Product Code	Brand	Quantity	Excitation/Emission	Conjugate
12770170	Invitrogen™ 88-8005-74	200 Tests	488, 535/520, 617	FITC, Propidium Iodide
15561187	Invitrogen™ 88-8006-72	50 Tests	405, 546/450, 647	eFluor 450, 7-AAD
15571187	Invitrogen™ 88-8006-74	200 Tests	405, 546/450, 647	eFluor 450, 7-AAD
12780170	Invitrogen™ 88-8007-74	200 Tests	650, 535/660, 617	APC, Propidium Iodide
15591187	Invitrogen™ 88-8008-72	50 Tests		PCP-eFluor 710
15413392	Invitrogen™ 88-8008-74	200 Test		PCP-eFluor 710
15581947	Invitrogen™ BMS500FI-100	100 Tests	488, 535/520, 617	FITC, Propidium Iodide
15501957	Invitrogen™ BMS500FI-20	20 Tests	488, 535/520, 617	FITC, Propidium Iodide
15521957	Invitrogen™ BMS500FI-300	300 Tests	488, 535/520, 617	FITC, Propidium Iodide
14819780	Invitrogen™ 88-8102-72	50 Tests		PE, 7-AAD
15521207	Invitrogen™ 88-8102-74	200 Tests		PE, 7-AAD
15531207	Invitrogen™ 88-8103-72	50 Tests		PE/Cy7
15541207	Invitrogen™ 88-8103-74	200 Tests		PE/Cy7
15551947	Invitrogen™ BMS500BT-100	100 Tests	NA, 535/NA, 617	Biotin, Propidium Iodide
15561947	Invitrogen™ BMS500BT-20	20 Tests	NA, 535/NA, 617	Biotin, Propidium Iodide
15571947	Invitrogen™ BMS500BT-300	300 Tests	NA, 535/NA, 617	Biotin, Propidium Iodide
12760170	Invitrogen™ 88-8005-72	50 Tests	488, 535/520, 617	FITC, Propidium Iodide
15581187	Invitrogen™ 88-8007-72	50 Tests	650, 535/660, 617	APC, Propidium Iodide

Description

Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.

Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.

In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).

Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.

Specifications

• Product Type: Apoptosis Detection Kit