Thermo Scientific™ EasyPep™ MS Sample Prep Kits

Generate high quality proteomic samples for MS analysis in less than four hours using optimized EasyPep MS Sample Prep kits.

Brand:  Thermo Scientific™ A40006

Product Code. 15947032

Description

EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than three hours. Kits are optimized to process protein samples from 10–100 μg with a high yield of MS-ready peptides.

• Complete—includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples
• Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
• Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
• Time-saving—sample processing time reduced from more than one day to less than three hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling

The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.

Specifications

• Description: Mini MS Sample Prep Kit
• Product Type: Sample Preparation Kit
• Shipping Condition: Wet Ice
• Final Product Type: Peptides
• Workflow Step: Cell Lysis

• For Use With (Equipment): Mass Spectrometer
• Product Line: EasyPep™
• Starting Material: Protein Samples
• Detection Method: Mass Spectrometry
• Quantity: 20 Reactions

Safety and Handling

Product Identifier

• 20 TEST EasyPep MiniMS Sample Prep Kit

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1A

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H312-Harmful in contact with skin.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : formic acid, tris(2-carboyethyl)phosphonium chloride, 2-chloroacetamide, acetonitrile, trypsin