Invitrogen™ Complement C5a Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ BMS2088

Product Code. 15314988

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human C5a

Description

The Human Complement C5a (Hu C5a) ELISA quantitates Hu C5a in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C5a. Principle of the method The Human C5a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9). The molecular mass of C5 is 190 kDa.

Specifications

• Accession Number: P01031
• Assay Sensitivity: 0.005 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: C5Da,C5b,CPAMD4,ECLZB,C5
• Intraassay CV: 3.6%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 30 min.

• Assay Range: 0.078-5.0 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 727
• Interassay CV: 4%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Plasma, 10 μL; Serum, 5 μL; Supernatant, 50 μL
• Synonym: complement component 5, C5a, C5D, C5b, ECLZB, CPAMD4
• Test Time: 1 hr. 20 min.