Invitrogen™ BAFF R (TNFRSF13C) Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMTNFRSF13C

Product Code. 15425343

Description

The Mouse BAFF R (TNFRSF13C) ELISA quantitates Ms BAFF R in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms BAFF R. Principle of the method The Mouse BAFF R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Specifications

• Accession Number: Q9D8D0
• Assay Sensitivity: 28 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 72049
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 28.67-8000 pg/mL
• Calibration Range: 28pg/mL to 7,000pg/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA kit shows no cross-reactivity with the following mouse cytokines tested: BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: 2010006P15Rik,BAFF-R,Baffr,Bcmd,Bcmd-1,Bcmd1,Lvis22
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Synonym: 2010006P15Rik, BAFF-R, Baffr, Bcmd, Bcmd-1, Bcmd1, Lvis22
• Test Time: 1 hr. 20 min.