MDM2 Monoclonal Antibody (IF2), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA1113

Product Code. 12831723

Description

This antibody recognizes the ∽90 kDa (apparent MW) MDM2 protein. Also recognizes isoforms at ∽57 and ∽74/76 kDa. This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.

Specifications

• Antigen: MDM2
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide; pH 7.2-7.4
• Gene Accession No.: Q00987
• Gene Symbols: MDM2
• Immunogen: Human MDM2
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Immunoprecipitation, Western Blot
• Clone: IF2
• Conjugate: Unconjugated
• Gene: MDM2
• Gene Alias: 1700007J15Rik; AA415488; ACTFS; double minute 2; double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; HGNC:6973; human homolog of; MDM2; Mdm-2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2 p53 binding protein homolog; MDM2 proto-oncogene; MDM2 proto-oncogene, E3 ubiquitin protein ligase; MDM2 variant FB28; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53 E3 ubiquitin protein ligase; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; transformed mouse 3T3 cell double minute 2
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 4193
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid