Huntingtin Monoclonal Antibody (4-13), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA1058

Product Code. 14878191

Description

MA1-058 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry on human and mouse samples. Neoepitope antibodies distinguish smaller cleaved fragments or processed forms of proteins versus the intact full-length or precursor by using a designed peptide purification process to maximize immunoreactivity to a specific cleavage site. Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586. Neo-specific antibody MA1-058 recognizes the 552 cleaved fragment without detecting the full-length form.

Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.

Specifications

• Antigen: Huntingtin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P42858, P42859
• Gene Symbols: Htt
• Immunogen: Synthetic peptide conjugated to KLH via cysteine corresponding to residues CSDPAMDLND (544-552) of Human HTT.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry, Western Blot
• Clone: 4-13
• Conjugate: Unconjugated
• Gene: Htt
• Gene Alias: AI256365; C430023I11Rik; Hd; HD protein; HD protein homolog; Hdh; HTT; Huntingtin; huntingtin (Huntington disease); Huntingtin, myristoylated N-terminal fragment; Huntington disease gene homolog; huntington disease protein; huntington disease protein homolog; IT15; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 15194, 3064
• Content And Storage: -20°C
• Form: Liquid