HSPA4 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA529484

Product Code. 13473627

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, U87-MG, SK-N-SH, IMR32, SK-N-AS. Predicted reactivity: Mouse (97%), Rat (96%), Xenopus laevis (87%), Dog (98%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.

Specifications

• Antigen: HSPA4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HSPA4
• Gene Alias: 70kDa; AI317151; APG2; APG-2; epididymis secretory sperm binding protein Li 5a; heat shock 70 kDa protein 4; heat shock 70kD protein 4; heat shock 70kDa protein 4; Heat shock 70-related protein APG-2; Heat shock protein; heat shock protein 4; heat shock protein Apg-2; heat shock protein family A (Hsp70) member 4; heat shock protein, 110 kDa; HEL-S-5a; HS24/P52; Hsp110; hsp70; hsp70 RY; Hsp70RY; Hspa4; HSPH2; Irp94; ischemia responsive 94 kDa protein; mKIAA4025; RY
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 266759, 3308
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O88600, P34932
• Gene Symbols: HSPA4
• Immunogen: Recombinant fragment corresponding to a region within amino acids 75 and 374 of Human HSP70 4
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Non-human Primate, Rat
• Product Type: Antibody
• Isotype: IgG