P-Glycoprotein Monoclonal Antibody (C219), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA126528

Product Code. 11589512

Description

Recommended positive controls: Cell lines or frozen tissue, such as liver or colon. MA1-26528 is expected to cross react with a wide range of mammals due to sequence homology. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. This clone recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, MDR1 P-glycoprotein (∽170kDa) and MDR3 P-glycoprotein (∽140kDa). In Western blot, boiling samples may reduce visibility of the protein. For flow cytometry, cells should be fixed in 3.7% formaldehyde for 10 min at room temperature to permeabilize the cell membrane. Incubate cells with 5-10 μg antibody for 30-60 min at 4oC.

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

Specifications

• Antigen: P-Glycoprotein
• Classification: Monoclonal
• Concentration: 0.72 mg/mL
• Formulation: PBS with 1% BSA and 0.1% sodium azide
• Gene Accession No.: P06795, P08183, P21447, P21448, P21449
• Gene Symbols: ABCB1, Abcb1a, Abcb1b
• Immunogen: SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.
• Quantity: 250 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Fish, Hamster, Human, Mouse, Rat, Zebrafish
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: C219
• Conjugate: Unconjugated
• Gene: ABCB1
• Gene Alias: ABC superfamily; P-glycoprotein; ABC20; ABCB1; Abcb1a; Abcb1b; Abcb4; ATP binding cassette subfamily B member 1; ATP-binding cassette sub-family B (MDR/TAP) member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette sub-family B member 1; ATP-binding cassette sub-family B member 1A; ATP-binding cassette sub-family B member 1B; ATP-binding cassette transporter subfamily B, member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette, subfamily B (MDR/TAP), member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 1A; ATP-binding cassette, subfamily B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B, member 1A; ATP-binding cassette, subfamily B, member 1B; ATP-dependent translocase ABCB1; ATP-dependent translocase ABCB1; multidrug r
• Host Species: Mouse
• Purification Method: purified
• Regulatory Status: RUO
• Gene ID (Entrez): 100682536, 100682537, 170913, 18669, 18671, 24646, 281585, 5243
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid