GAS2L1 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA521729

Product Code. 13239918

Description

Recommended positive controls: A431, HeLa, A375, mouse brain. Predicted reactivity: Mouse (86%), Rat (86%), Pig (91%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The Gas2 protein is thought to play a role in apoptosis by acting as a cell death substrate for caspases. Gas2, a component of the microfilament system, is cleaved by a caspase (caspase-3 or caspase-7) at Asparagine 278 during apoptosis. The cleaved form of Gas2 dramatically induces the re-arrangement of the Actin cytoskeleton and causes potent changes in the shape of the affected cells. Gas2 is a cytoskeleton and peripheral membrane protein that co-localizes with Actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Gas2 is mainly membrane-associated, but when hyperphosphorylated it will accumulate at membrane ruffles, indicating Gas2 involvement in that process. Gar22 (Gas2-related protein on chromosome 22), also designated growth arrest-specific protein 2-like 1 (Gas2-like1), belongs to the Gas2 family and contains one CH (calponinhomo-logy) domain. It is thought to be involved in the cross-linking of microtubules and microfilaments.

Specifications

• Antigen: GAS2L1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GAS2L1
• Gene Alias: 4930500E24Rik; D0Jmb1; Gar22; GAS2L1; GAS2-like protein 1; GAS2-related protein; GAS2-related protein on chromosome 22; growth arrest specific 2 like 1; growth arrest-specific 2 like 1; growth arrest-specific protein 2-like 1; TU-71.1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10634, 78926
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.8 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q8JZP9, Q99501
• Gene Symbols: GAS2L1
• Immunogen: Recombinant fragment corresponding to a region within amino acids 71 and 355 of GAS2L1 (Uniprot ID#Q99501)
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG