MTCO1 Monoclonal Antibody (1D6E1A8), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 459600

Product Code. 10444035

Description

For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal. Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio. In Western blot applications, this antibody detects a band at approximately 40 kDa (predicted MW: 57 kDa). In mouse liver lysate a specific band below 37 kDa was detected. Recommended positive controls: ICC/IF - Pig retinal pigment epithelial cells, rat cerebellum primary cells IHC (P) - Human skeletal muscle and colon tissue, rat pancreas tissue, pig smooth muscle tissue WB - Mouse, human, bovine and rat heart mitochondria lysate Flow - HEK-293 cells Recommended long term storage at -20°C.

MTCO1 Monoclonal antibody specifically detects MTCO1 in Human, Mouse, Rat samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot.

Specifications

• Antigen: MTCO1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 59% PBS with 0.05% BSA, 40% glycerol and 0.01% sodium azide
• Gene Accession No.: P05503, P00395, Q36347, O79876, P00397, Q9MIY8
• Gene Symbols: COX1, MT-CO1
• Immunogen: Human Mitochondrial Complex IV subunit I
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Clone: 1D6E1A8
• Conjugate: Unconjugated
• Gene: MT-CO1
• Gene Alias: Co1; coi; cox1; coxi; Cytochrome c oxidase polypeptide I; Cytochrome c oxidase subunit 1; cytochrome c oxidase subunit I; mitochondrially encoded cytochrome c oxidase I; mtco1; mt-co1
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 140539, 17708, 26195, 4512, 1485858, 808503
• Content And Storage: 4° C, do not freeze
• Form: Liquid