alpha Tubulin Monoclonal Antibody (B-5-1-2), Alexa Fluor™ 488, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 322588

Product Code. 10585104

Description

This antibody reacts with all forms of alpha tubulin. The expected molecular weight is ∽50.5 kDa. Reactivity is confirmed with NIH3T3 cells, rat brain, mouse testis, human Caco-2, HeLa3 and PtK23 cells. Excitation/Emission (nm) for this Alexa Fluor 488™-conjugated antibody: 495/519

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

Specifications

• Antigen: alpha Tubulin
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 4mg/mL BSA and 0.1% sodium azide; pH 7.4
• Gene Accession No.: A6NHL2, P05213, P0DPH7, P68363, P68366, P68368, P68369, P68370, P68373, Q3KRE8, Q3UX10, Q5XIF6, Q6AYZ1, Q6P9V9, Q6PEY2, Q71U36, Q9BQE3, Q9BVA1, Q9CWF2
• Gene Symbols: Tuba1a, Tuba1b, TUBA1C, TUBA3C, TUBA3E, TUBA4A, TUBAL3, TUBB2B
• Immunogen: Sarkosyl-resistant filament from sea urchin sperm axonemes
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Sea urchin
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunocytochemistry
• Clone: B-5-1-2
• Conjugate: Alexa Fluor 488
• Gene: TUBAL3
• Gene Alias: &agr-tubulin 84B; 1t; a1t; ALPHA 84C; alpha tubulin; alpha tubulin 84B; alpha[[1]] tubulin; alpha[[1]]-tubulin; alpha1; alpha1 tubulin; alpha1t; alpha1tub; alpha1tub84B; alpha1-tubulin; alpha84B; alphaT; alphat-1; alphatub; alpha-tub; alphaTub1; alphatub84; alphaTUB84B; alpha-tub84B; alphaTub84B-PA; alphatubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; alpha-tubulin 4; Alpha-tubulin 6; alphaTubulin 84B; alpha-Tubulin 84B; alpha-Tubulin at 84B; alpha-tubulin I; Alpha-tubulin II; alpha-tubulin III; Alpha-tubulin isotype M-alpha-1; alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; alpha-tubulin isotype M-alpha-6; alpha-tubulin TUB1; alpha-tubulin ubiquitous; alphaTubulin84B; Alpha-Tubulin84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; AT25469p; aTub84B; a-tub84B; bA408E5.3; B-ALPHA-1; bcm948; BEST:LD32507; cb944; CG1913; CG1913-PA; chr3R:2914276..2914416; clone 1.59; c
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 10376, 112714, 22142, 22143, 22145, 22146, 238463, 291081, 291287, 300218, 316531, 347733, 500929, 64158, 7277, 7278, 73710, 7846, 79861, 84790
• Content And Storage: Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
• Form: Liquid