Invitrogen™ TRI Reagent™ Solution

Tri Reagent™ solution is a complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.

Brand:  Invitrogen™ AM9738

Product Code. 11312940

Description

Tri Reagent™ solution is a complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. Key features:

• Simple—a single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein
• Productive—obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods
• Versatile—works with many sample sources, including human, plant, yeast, bacterial and viral samples
• Flexible—easily scalable depending on starting material and desired yield

Using TRI Reagent™ solution
TRI Reagent™ starting material can be either fresh or frozen tissue, or cell samples of almost any size—the protocol is easily scalable. This RNA isolation method is well-recognized and referenced in numerous publications, a testament to its reliability.

How it works
TRI Reagent™ combines phenol and guanidine thiocyanate in a monophase solution to facilitate the immediate inhibition of RNase activity. Biological samples are homogenized or lysed in TRI Reagent™; the subsequent addition of bromochloropropane or chloroform results in the separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase. The RNA can then be precipitated from the aqueous phase with the addition of isopropanol. The entire protocol can be completed in approximately 1 hour.

Applications
The isolated RNA is suitable for any downstream application, including RT-PCR, amplification for array analysis, hybridization assays, or in vitro translation. PCR, Southern blotting, and cloning can be performed using DNA isolated with TRI Reagent™. Isolated proteins can be used in SDS-PAGE and western blotting.

Tri Reagent™ solution is incorporated into our RiboPure™ and MagMAX™-96 for Microarrays RNA isolation kits.

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: 100 mL TRI Reagent; 4°C
  
  
• Purification Time: 1 hr.
• Sample Type: Bacteria, Cells, Plants, Tissue, Yeast
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 100 mL
• Starting Material Amount: Bacteria: ≤10⁷ cells
  Cells: ≤10⁶
  Plant: ≤10⁶ cells
  Tissue: ≤100 mg
  Yeast: ≤10⁶ cells

• Isolation Technology: Organic Extraction
• Elution Volume: Varies
• Final Product Type: Total RNA, DNA, Protein
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
• Shipping Condition: Room Temperature
• Yield: RNA: ≤15 μg (cells), ≤10 μg (tissue)

Safety and Handling

Product Identifier

• TRI Reagent Solution

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• LONG-TERM AQUATIC HAZARD Chronic 3
• Germ cell mutagenicity Category 2
• Skin corrosion/irritation Category 1 B
• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H301-Toxic if swallowed.
• H311-Toxic in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H331-Toxic if inhaled.
• H341-Suspected of causing genetic defects.
• H373-May cause damage to organs.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : phenol, thiocyanic acid

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For Research Use Only. Not for use in diagnostic procedures.