Invitrogen™ TaqMan™ PreAmp Cells-to-C_T™ Kit

The TaqMan PreAmp Cells-to-C_T Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.

Brand:  Invitrogen™ 4387299

Product Code. 10490345

Includes: 40 sample prep rxns, 40 Dnase rxns, 40 reverse transcription rxns, and 100 20μL PCR rxns for 40 reactions.

Description

• Complete, validated solution: Optimized workflow includes cell lysis reagents, DNase, RT reagents, PreAmp reagents, and TAqMan Gene Expression Master Mix
• Fast, simple and convenient: Prepare samples in 7 minutes; eliminate tedious RNA isolation
• Extend limited samples: Increase real-time PCR potential by up to 64-fold; study up to 100 genes at once
• Accurate results: Uniform amplification without introducing bias
• Robust performance: Linear detection from 10 to 100,000 cells; results equivalent to purified RNA
• Fewer resources used, less hazardous, less waste

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage:

  • 40 x 20 μL sample prep reactions
  • 40 x 20 μL Dnase reactions
  • 40 x 20 μL reverse transcription reactions
  • 100 x 20 μL PCR reactions

• Format: Kit
• PCR Method: 2-step RT-qPCR
• Reaction Speed: Standard
• Green Features: Fewer resources used and less waste, Less hazardous
• Product Line: Ambion™, Cells-to-CT™, TaqMan™
• Purity or Quality Grade: UP (Ultra Pure)
• Reverse Transcriptase: M-MLV
• Sufficient For: 40 Reactions
• No. of Reactions: 40 Reactions

• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• Polymerase: DNA Polymerase
• For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Preamp Cells-to-C Kit
• Quantity: 40 reactions
• Shipping Condition: Dry Ice
• For Use With (Application): Pre-amplification
• Sample Type: Cell

For Research Use Only. Not for use in diagnostic procedures.