Invitrogen™ PureLink™ RNA Mini Kit

The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment.

Brand:  Invitrogen™ 12183020

Código de producto. 10359103

Descripción

Rapid and convenient column purification
The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000 μg of purified RNA from a single extraction. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.

Simplified, nontoxic RNA purification
The PureLink™ RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.

• Obtain high-yield, high-integrity RNA
• Rapid, non-hazardous RNA purification capabilities
• Ready-to-use RNA for many downstream applications

Especificaciones

• Content And Storage: • 20 mL Lysis Buffer; store at room temperature
  • 10 mL Wash Buffer I; room temperature
  • 4 mL Wash Buffer II; room temperature
  • 3 mL RNase-free Water; room temperature
  • 10 PureLink Spin Cartridges with Collection Tubes; room temperature
  • 10 Collection Tubes; room temperature
  • 10 Recovery Tubes; room temperature
• Purification Time: 20 min. (After sample homogenization)
• Sample Type: Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 10 Preps
• Shipping Condition: Room Temperature
• Yield: Up to 350 μg

• Isolation Technology: Silica Spin Column
• Elution Volume: 30–300 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Scale: Mini
• Starting Material Amount: Bacteria: ≤10⁹ cells
  Blood: ≤0.2 mL
  Cells: ≤5 x 10⁷
  Plant: ≤250 mg
  Tissue: ≤200 mg
  Yeast: ≤5 x 10⁸ cells
  Enzymatic reactions: ≤1.2 mL

Seguridad y manipulación

Nombre del producto

• PureLink RNA Mini Kit

Palabra de advertencia

• Danger

Clasificación

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 1 C
• Skin corrosion/irritation Category 2

Indicaciones de peligro

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H412-Harmful to aquatic life with long lasting effects.

Consejos de prudencia

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Otros peligros

• MIXTURE LIST-Contains : Thiocyanic acid

For Research Use Only. Not for use in diagnostic procedures.